Extended Data Fig. 9: Neuronal and adipocyte populations.
a, UMAP embedding identifies six neuronal subpopulations. b, Regional distribution of neuronal cell subpopulations identified in a. Data are available in Supplementary Table 20. c, Expression (log2FC) dot plot of selected marker genes in neuronal cell subpopulations. d, Multiplexed smFISH of NRXN1 (green) and PPP2R2B (red), nuclei were DAPI-stained (dark blue). Scale bars, 5 μm. For details on statistics and reproducibility, see Methods. e, UMAP embedding showing four adipocyte subpopulations. f, UMAP embedding of expression of gene markers associated with adipocytes (GPAM, FASN, ADIPOQ, LEP). g, Top five significantly enriched pathways for each adipocyte subpopulation, using differentially expressed genes calculated using the Wilcoxon rank sum test with Benjamini–Hochberg correction (logFC >0.5, P < 1.0 × 10−5) and tested using a hypergeometric distribution with Bonferroni correction as implemented in ToppFun. Data are available in Supplementary Table 21. h, Expression (log2FC) of adipocyte associated genes and select marker genes from the top enriched pathway for each adipocyte subpopulation.
