Extended Data Fig. 3: Enhancer reporter assay for tissue-specific enhancers.
From: A map of cis-regulatory elements and 3D genome structures in zebrafish
In total, 28 of 32 predicted tissue-specific enhancers showed consistent GFP signals in the corresponding tissues. For the eight brain enhancers tested, 63/95, 51/86, 85/119, 112/143, 27/45, 34/48, 27/41, 62/77, and 37/45 embryos, respectively, had green signals in the brain region. For the six tested heart enhancers, 64/94, 52/85, 79/121, 20/41, 51/95, 32/55 and 20/31 embryos, respectively, had green signals in the heart region. For the six tested muscle enhancers, 52/57, 26/30, 107/124, 53/63, 93/114, 61/67 and 66/78 embryos, respectively, had green signals in the trunk muscle. For the four selected kidney enhancers, 47/82, 35/67, 44/62, 15/42 and 56/110 embryos, respectively, had green signals in the kidney region.
