Extended Data Fig. 5: Split-GAL4 driver lines targeting vpoENs and vpoINs and neurotransmitter types revealed by FISH. | Nature

Extended Data Fig. 5: Split-GAL4 driver lines targeting vpoENs and vpoINs and neurotransmitter types revealed by FISH.

From: Neural circuit mechanisms of sexual receptivity in Drosophila females

Extended Data Fig. 5

a, Confocal images of female central nervous system carrying indicated split-GAL4 driver lines and UAS-myrFLAG or UAS-Chrimson-mVenus. Samples were stained with anti-FLAG or anti-GFP (green) to reveal membranes of targeted neurons and mAb nc82 to reveal all synapses (magenta). Arrows indicate soma. Scale bar: 100 μm. b, Confocal images showing the expression of ChAT, GAD1, and vGluT in vpoEN and vpoIN neurons (labelled by Halo tag, arrows) in female brains, as revealed by FISH. Representative images are shown from at least 5 independent samples examined in each case.

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