Extended Data Fig. 7: Cells that escape Notum deletion upregulate Wif1, but not Dkk3.
From: NOTUM from Apc-mutant cells biases clonal competition to initiate cancer
a, Quantification of the relative percentage of Notum+ adenomas (as detected via Notum-ISH) in VilCreER;ApcMin/+;Notum+/+ (+/+) and VilCreER;ApcMin/+;Notumfl/fl (fl/fl) mice aged 85 days. Mice were induced with 2 mg tamoxifen at 6 and 8 weeks of age. n = 11 Notum+/+ and n = 8 Notumfl/fl mice. b, Representative RFP-IHC of VilCreER;ApcMin/+;tdTom+ tumour tissue to show recombination efficiency at 85 days of age. Mice were induced with 2 mg tamoxifen at 6 and 8 weeks of age. The boxed area shows a close-up of recombined (dark brown cells) and non-recombined (light brown cells) tumour epithelium from a single VilCreER;ApcMin/+;tdTom+ mouse. Sections from n = 4 mice were stained. Scale bar, 10 mm. c, Serial sections of intestinal tumour tissue stained via ISH for Notum, Wif1 and Dkk3 from two separate VilCreER;ApcMin/+;Notumfl/fl mice described in a. Note, Wif1 is upregulated in Notum-negative epithelial cells (boxed area). Scale bar, 50 μm. Data are mean ± s.e.m.
