Extended Data Fig. 10: BirA*–Flag and GFP–BirA*–Flag control stable cell line, and LMNA-BirA*–Flag and AIFM1-BirA*–Flag bait stable cell line immunofluorescence.
From: A proximity-dependent biotinylation map of a human cell
Cell lines were probed by confocal immunofluorescence microscopy in HEK293 Flp-In T-REx stable cells to assay for localization of the fusion construct and general biotinylation. Cells were fixed and then probed with an antibody to the Flag epitope and streptavidin for biotinylated proteins (Methods). The green channel represents nuclear or mitochondrial staining, the red channel denotes Flag and the blue channel represents streptavidin (biotinylated proteins). Scale bars, 10 μm.
