Extended Data Fig. 3: Flow cytometry.
From: Naturally enhanced neutralizing breadth against SARS-CoV-2 one year after infection
a, Gating strategy. Gating was on singlets that were CD20+ and CD3-CD8-CD16-Ova-. Anti-IgG, IgM, and IgA antibodies were used for B cell phenotype analysis. Sorted cells were RBD-PE+ and RBD/KEN-AF647+. b, c, Flow cytometry showing the percentage of RBD-double positive (b) and 647-K417N/E484K/N501Y mutant RBD cross-reactive (c) memory B cells from 1.3 or 6- and 12-months post-infection in 10 selected participants. d, As in Fig. 2b, Pie charts show the distribution of antibody sequences from 4 individuals after 1.33 (upper panel) or 6.24 months (middle panel) or 12 months (lower panel). e, Circos plot depicts the relationship between antibodies that share V and J gene segment sequences at both IGH and IGL. Purple, green, and grey lines connect related clones, clones and singles, and singles to each other, respectively. f, Graph summarizes cell number (indicated in b, c) (per 2 million B cells) of immunoglobulin class of antigens binding memory B cells in samples obtained at 1.3, 6.2 and 12 months. Each dot is one individual. (Vaccinees, n = 20, and non-vaccinees, n = 20). Red horizontal bars indicate mean values. Statistical significance was determined using two-sided Kruskal–Wallis test with subsequent Dunn’s multiple comparisons.
