Extended Data Fig. 9: Semi-continuous cultivation of the ‘Ca. Methanoliparum’ cultures at 55 °C.
From: Non-syntrophic methanogenic hydrocarbon degradation by an archaeal species
Microorganisms were cultured using a mixture of n-docosane, n-hexadecyl benzene, n-hexadecyl cyclohexane as substrate. The culture was transferred when 15 to 20 mmol of methane were formed, and 30% to 50% of the culture were transferred. Displayed are transfers 3 to 6. a, Methane formation in the headspace. Grey arrows indicate transfer events. b and c, Abundance of 16S rRNA gene of ‘Ca. Methanoliparum’ and bacteria as determined by qPCR, respectively. d, Relative abundance of main archaeal groups determined by 16S rRNA gene sequencing with archaeal primer set Arch519F/Arch915R.
