Extended Data Fig. 8: Examples of leader-containing reads for Orf9b and N from the RNA-seq dataset.
From: Evolution of enhanced innate immune evasion by SARS-CoV-2
a–c, Representative sequence for Orf9b (top) and N (bottom) sgRNA from Alpha (a), VIC (b) and IC19 (c). Leader sequences to identify sgRNAs are highlighted in yellow. The following sequence is used to differentiate Orf9b versus N sgRNAs. Orf9b and N start codons shown in maroon. The site of the N-protein D3L mutation is indicated in green, resulting in increased similarity to the transcriptional regulatory sequence (TRS) for Alpha. Read counts of Orf9b and N are indicated to the right. Counts are normalized to mean genomic reads per replicate.
