Extended Data Fig. 8: Biological impacts of DOCK2 downregulation in primary cells and DOCK2 knockdown and Interferon-α production assay in THP-1 Blue ISG cells.

(a) The impact of DOCK2 downregulation on interferon-α (IFN-α) production ability in pDC. Sorted pDC were stimulated with CpG and/or CPYPP. Data shows means ± s.e.m. (n = 3 per group). Differences of IFN-α production ability between the groups were evaluated using two-sided paired t-test. (b) The impact of DOCK2 downregulation on chemotaxis in CD3⁺ T cells. CD3⁺ T cells were stimulated with CXCL12 or CXCL12 + CPYPP (n = 19 per group). Differences of chemotaxis between the groups were evaluated using two-sided paired t-test. (c, d) Knockdown of DOCK2 by CRISPR system was confirmed by western blotting (c) and qRT-PCR. (d) Semi-quantitative staining density measure was determined using ImageJ (NIH). Data shows means ± s.e.m. (n = 3 per group). Data are compared to control group. P values were determined with One-way ANOVA followed by Dunnett’s post hoc test. (e, f) Activity ratio of SEAP reporter to no treatment group. Reporter was activated by 50 ng/ml LPS (e) or 50 μg/ml polyIC (f). Data shows means ± s.e.m. (n = 3 per group). Data are compared to control group. P values were determined with One-way ANOVA followed by Dunnett’s post hoc test.