Extended Data Fig. 1: Analysis of hrg-9 and hrg-10 expression in C. elegans.
From: HRG-9 homologues regulate haem trafficking from haem-enriched compartments
a, Workflow of RNA sequencing experiment. Synchronized L1 worms were grown in mCeHR2 medium containing 2 μM, 20 μM, or 400 μM haem to the gravid stage, and the intestines were isolated for transcriptomics analysis using the SMART-Seq technology. b, The hrg-9p::nls-gfp transcriptional reporter is expressed in the intestine of C. elegans at all developmental stages. nls, nuclear localization signal; i, intestine. Scale bars, 10 μm. c, The hrg-10p::nls-gfp transcriptional reporter is predominantly expressed in the intestine of C. elegans at all developmental stages. nls, nuclear localization signal; i, intestine. Scale bars, 10 μm. d, Knockdown of hrg-9 and hrg-10 by RNAi does not impair the intestinal secretion of YP170::GFP in C. elegans. The known trafficking gene rab-10 is used as a control. i, intestine; o, oocyte; e, embryo. Scale bars, 20 μm. e, RNA-seq demonstrates that intestinal hrg-10 expression is not significantly affected by haem levels in C. elegans. n = 3 independent samples for each group. f, Quantitative RT-PCR shows that haem does not regulate hrg-10 expression at the organismal level in C. elegans. n = 3 independent experiments. g, Haem does not regulate the expression of hrg-10p::nls-gfp transcriptional reporter. Scale bars, 10 μm. Data in e and f are presented as mean ± s.e.m. Statistical significance was determined by one-way ANOVA followed by Tukey’s test.
