Extended Data Fig. 6: (related to Fig 3): ILC2s regulate myeloid cells in type-2 inflammatory allergy models of the lung.
From: Non-redundant functions of group 2 innate lymphoid cells
a—d, Flow-cytometric quantification of cell population in Nmur1iCre-eGFP Id2fl/fl mice and littermate controls sensitized with A. alternata extract for three consecutive days, analysed 7 days after the first dose. a, Absolute numbers of lung ILC2s, lung and BAL eosinophils and total CD45+ cells in the BAL. b, Relative and absolute numbers of TH2 cells in the lung. c,d, relative (c) and absolute (d) numbers of myeloid populations from the lung interstitium, and alveolar macrophages from the BAL. Data in a—d are representative of two independent experiments with 4–6 animals per group. e—i, Analysis of Nmur1iCre-eGFP Id2fl/fl mice and littermate controls sensitized with papain for three consecutive days, analysed 7 days after the first dose. e, Absolute numbers of ILC2s in the lung interstitium, and eosinophils in the lung interstitium and BAL. f, Eosinophils are visualized by modified Sirius red stain in the lung. g, Relative and absolute numbers of TH2 cells in the lung. h,i, relative (h) and absolute (i) numbers of myeloid populations from the lung interstitium, and alveolar macrophages from the BAL. Data in e and g-i are pooled from two independent experiments with 4-5 mice per group. Untreated controls for (e-i) were Id2fl/fl, papain-treated littermate controls were Id2fl/fl, Id2fl/+ or Nmur1iCre-eGFP Id2fl/+. One symbol represents data from one mouse, data are mean ± s.d., One-way ANOVA with Tukey’s multiple comparison tests, NS: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. BAL, bronchoalveolar lavage.
