Extended Data Fig. 8: (related to Fig 3): Eosinophils are altered in ILC2-deficient mice.
From: Non-redundant functions of group 2 innate lymphoid cells
a—f, Flow-cytometric analysis of eosinophils and ILC2s in the BAL and lung interstitium of Id2fl/fl, Nmur1iCre-eGFP Id2fl/fl mice treated with either PBS or recombinant IL-5, seven days after allergy induction by intranasal application of A. alternata extract. a,b Eosinophils in the lung interstitium, c,d Eosinophils in the BAL, e,f ILC2s in the lung interstitium. Symbols represent data from one mouse, data are pooled from two independent experiments. Data are mean ± s.d., Student’s t-test, NS not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. g Flow cytometry plots showing the gating strategy for the analysis and sorting of eosinophils from lung after Papain treatment of Nmur1iCre-eGFP Id2fl/fl mice (cKO) and littermate controls (Ctrl). Data are representative of one experiment with 5 mice per group. h, Pathways from tmod gene set enrichment analysis (GSEA) of bulk RNA sequencing from eosinophils of 4 Nmur1iCre-eGFP Id2fl/fl mice vs 4 WT control animals. Bars are color-coded according to Databases: Gene Ontology (GO, red), Reactome (gray), Hallmark (white), tmod (purple), KEGG (pink). Genes differentially expressed in eosinophils after papain treatment of Nmur1iCre-eGFP Id2fl/fl mice (cKO) and littermate controls (Ctrl) were used for the analysis. i, Volcano plot showing differentially expressed genes of interest in eosinophils after papain treatment of Nmur1iCre-eGFP Id2fl/fl mice (cKO) and littermate controls (Ctrl) j, Heat maps of genes differentially expressed in eosinophils after papain treatment of Nmur1iCre-eGFP Id2fl/fl mice (cKO) and littermate controls (Ctrl). Each heat map corresponds to one pathway shown in h.
