Extended Data Fig. 1: (related to Fig 1): Gating strategy for flow cytometry.
From: Non-redundant functions of group 2 innate lymphoid cells
a, Generation of genetic construct of the Nmur1iCre–eGFP BAC transgene. 70th bp of exon 2 to 29th bp downstream of the TGA stop codon of exon 3 was replaced by a cassette containing iCre and eGFP linked with P2A and T2A. (86,388,138-86,386,296) were replaced through homologous recombination. The FRT-flanked kanamycin selection cassette was deleted after FLP-mediated recombination. Removal of LoxP and LoxP511 sites on BAC vector backbone. b, Expression of the Nmur1 gene in NMUR1–eGFP+ and NMUR1–eGFP− cells purified by FACsorting from the intestinal lamina propria. c, Gating strategy of bone marrow precursors. Lineage: CD3, CD5, CD19, Ly6G, FcεRIa. d, Gating strategy of intestinal lymphoid populations. e, Gating strategy of intestinal myeloid populations. Lineage: CD3, CD5, CD19, FcεRIa. f, Gating strategy of intestinal eosinophils, 2 alternative gates shown. Lineage: CD3, CD5, CD19, Ly6G. g, Gating strategy of γδ T cells, regulatory T cells (Treg) and TH2 cells from the mesenteric lymph node. h, Gating strategy of mast cells and basophils from the peritoneal lavage. Lineage: CD3, CD5, CD19, Ly6G.
