Extended Data Fig. 2: (related to Fig 1): Nmur1^iCre-eGFP specifically labels ILC2s in steady state and during type 2 inflammation.

a, Flow cytometry plots of GFP and ST2 in lung ILC2s (left) and T_H2 cells (right) of Nmur1^iCre-eGFP mice. Numbers denote percentage of cells inside the gate. b, GFP expression in T_H2 cells and ILC2s of Nmur1^iCre-eGFP mice, quantification of a. c, GFP expression in mLN CD45⁻ cells (black) and ILC2s (gray). d, Quantification of c. e, Quantification of GFP expression across organs and cell types as displayed in the heat map of Fig. 1d. f, RFP and ST2 in lung ILC2s (left) and T_H2 cells (right) of Nmur1^iCre-eGFP x Rosa26^LSL-RFP F1 offspring. Numbers denote percentage of cells inside the gate. g, RFP expression in T_H2 cells and ILC2s, quantification of f. h, RFP expression in mLN CD45⁻ cells (black) and ILC2s (gray) of Nmur1^iCre-eGFP x Rosa26^LSL-RFP F1 offspring. i, Quantification of h. j, RFP expression across organs and cell types as displayed in the heat map of Fig. 1h. k, Heat map of flow-cytometric quantification of GFP and RFP expression in T_H2 and T_reg cells. Values represent median percentage of positive cells of parental gate, n = 3 mice per group. l–q, Immunofluorescence micrographs of RFP expression in Nmur1^iCre-eGFP x Rosa26^LSL-RFP F1 offspring stained for alpha smooth muscle actin (α-SMA), CD45 and DAPI in the small intestine (l), mesenteric lymph nodes (m), lung (n), mesenteric fat (o), femoral muscle (p) and large intestine (q). r, GFP and Cre (RFP) expression seven days after Nippostrongylus brasiliensis (N.b.) helminth infection across immune cell populations in Nmur1^iCre-eGFP x Rosa26^LFL-RFP F1 offspring. s,t, GFP (s) and RFP (t) expression in Nmur1^iCre-eGFP Rosa26^LSL-RFP mice, seven days after infection with N.b.. Data in (r-t) are representative of two independent experiments with 3 mice per group.

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