Extended Data Fig. 2

TSWV infection or overexpression of NSs in Arabidopsis affect JA, AUX and SL signalling pathways. a, Analysis of published transcriptome data⁵⁰ showed that the majority of JA, AUX and SL responses genes are downregulated in Arabidopsis plants infected with TSWV at 9, 12 and 15 dpi. Gene expression up- or down-regulated (with at least a two-fold difference) are presented in red and green, respectively. b-d, Relative expression levels of JA, AUX and SL responses genes in Arabidopsis plants infected with TSWV at 12 dpi. Data are represented as means ± s.e.m.; n = 3 biologically independent samples. e, Amount of jasmonic acid (JA), indole-3-acetic acid (IAA) and 5-deoxystrigol (5-DS) in Arabidopsis plants infected with TSWV at 12 dpi. The Arabidopsis plants inoculated with 1×PBS buffer were used as Mock. Data are represented as mean values ± s.e.m.; n = 3 biologically independent samples. f, Transgenic Arabidopsis lines expressing NSs (1 to 8) were screened and examined by RT-PCR (top). Phenotypes of wild-type (WT) and NSs transgenic line #2 and line #3 Arabidopsis plants are shown at the bottom. g, The NSs transgenic plants are less sensitive to methyl jasmonate (MeJA)-induced root growth inhibition than WT plants. NSs transgenic line #2 (left) and #3 (right) were treated with MeJA. The photograph was taken 21 days post MeJA treatment. Scale bar, 1 cm. h, Relative expression levels of representative JA response genes in WT and NSs transgenic Arabidopsis plants in the presence of 0 or 100 µM MeJA. Data are represented as mean values ± s.e.m.; n = 3 biologically independent samples. i, Illustration of the experimental design for thrips attraction to WT and NSs transgenic plants. The NSs transgenic plants were placed at two corners across each other (i and iii) and WT plants placed in the other two corners (ii and iv) in an isolated box. The western thrips without carrying TSWV were placed in a petri dish in the center of the box. The thrips attracted onto WT and NSs transgenic plants were counted after the indicated time and shown in Fig. 1c. j, The NSs transgenic plants are less sensitive to auxin analog 2,4-D-induced root growth inhibition than WT plants. The photograph was taken at 14 days post 2,4-D treatment. Scale bar, 1 cm. k, Primary root lengths of 7-day-old WT and NSs transgenic Arabidopsis plants with or without 1 μM rac-GR24. Data are presented as mean values ± s.e.m.; n = 12 plants. Data in (b-e, h and k) were analysed by two-sided Student’s t-test; P values are shown in the figure. Experiments were repeated at least three times with similar results.

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