Extended Data Fig. 5: Conservation of gene expression trajectories along spermatogenesis.
From: The molecular evolution of spermatogenesis across mammals
a, Global trajectory conservation score across amniotes for changed (n = 237) and conserved (n = 389) trajectories (P < 2.2x10−16, two-sided Wilcoxon rank-sum test). b, Trajectory conservation score between human and mouse of genes that lead to either a fertile (n = 1077) or an infertile (n = 108) phenotype when knocked out in mouse (P = 0.007, two-sided Wilcoxon rank-sum test). a,b, Box plots depict the median (center value); upper and lower quartile (box limits) with whiskers at 1.5 times the interquartile range. c, Gene expression trajectories (DMRT1, top; IP6K1, bottom) along primate spermatogenesis (spermatogenesis, from left to right). For each replicate, mean expression levels across cells of a given cell type were calculated. Marks indicate values for the replicates with the highest and lowest mean expression levels, dots indicate the median of mean expression values of the replicates. There are three replicates for chimpanzee; two replicates for human, bonobo, gorilla, macaque, and marmoset; one replicate for gibbon. The dashed vertical lines separate spermatogonia (SG), spermatocytes (SC), round (rSD) and elongated spermatids (eSD).
