Extended Data Fig. 6: A mutation that disrupts inter-oligomeric polymerization blocks septin localization to the cell surface.
From: Blebs promote cell survival by assembling oncogenic signalling hubs
Top, a cartoon illustrating the mechanism underlying the altered function of the SEPT2(33–306) mutant. Bottom left above, mean local SEPT6-GFP intensity and intracellular mean curvature as a function of distance from bleb edges in cells expressing SEPT2(33–306), calculated from 1293 blebs across 8 MV3 cells. Error bands indicate 95% confidence intervals. Bottom left below, difference in mean local SEPT6-GFP intensity compared to local maxima as a function of distance from bleb edge for cells expressing SEPT2(33–306) and control cells (as seen in Fig. 2e). Bottom right, Western blot of SEPT2 in MV3 cells with and without SEPT2(33–306) expression. Calculated mass of SEPT2 is 41.5kD and SEPT2(33–306) is 31.7 kD. Annotations show positions of the indicated ladder bands.
