Fig. 2: PET-guided multi-modality imaging to characterize spatial architecture of mitochondrial networks in NSCLC.
From: Spatial mapping of mitochondrial networks and bioenergetics in lung cancer
a, Schematic of multi-modality imaging technique and analysis approach. b, [18F]FDG transverse (left) and 3D-rendered (right) PET–CT images. [18F]FDGHI tumour (KL) was identified with heterogeneous regions of high and low [18F]FDG uptake. Scale bar, 5 mm. c, 3D-rendered microCT image of isolated lung lobe with tumour in b. Dashed line represents the orientation of sectioning plane on the tumour. Scale bar, 5 mm. d, Left: high-resolution microCT images on heavy-metal-stained tumour block. Selected region for SBEM imaging is indicated by white arrowhead. Scale bars, 500 μm (top) and 1 mm (bottom). Right: representative subvolume of SBEM images. Scale bar, 10 μm. e, The landscape of SBEM-imaged OXPHOSLO LUSC tumour volume after individual cell segmentation and cell-type classification. LUSC, blue; neutrophil (NTPH), yellow; red blood cell (RBC), purple; LUAD, red. f, Quantification of different cell types in e.
