Extended Data Fig. 1: Variant validation and protein expression of AR and DAAM2 in male control GSF and both mutant strains.
From: Formin-mediated nuclear actin at androgen receptors promotes transcription
a Sanger Validation of the variants found in DAAM2 mutant strains GSF-104 (NM_001201427.1 c.1840_1841insC) and GSF-163 (NM_001201427.1 c.495C>G). b DAAM2 knock down efficiency in male control GSF-22 at the mRNA and c protein expression level. d DAAM2 mRNA expression levels in male control GSF and both mutant strains. e Western blots showing whole cell extract expressions of AR and DAAM2 in two different scrotal (GSF-22 and GSF-26) and two different foreskin controls (GSF-122 and GSF-128) together with the DAAM2 mutants (GSF-104 and GSF-163). Ponceau staining served as loading control. f Quantification of DAAM2 protein levels normalized to ponceau. g Quantification of AR protein levels normalized to ponceau. The experiments were performed in three independent biological replicates. Means +/− SD are shown. P-values were calculated using a two-sided t-test. * p = 0.022, ** p = 0.0034, **** p < 0001. Values of p < 0001 are denoted by four stars, values of p < 001 are denoted by three stars, values of p < 005 are denoted by two stars, and values of p < 05 are denoted by one star. h Cytoplasmic (CE) and nuclear (NE) expression of AR, DAAM2 in male control GSF (GSF-26 and GSF-122) and both mutant strains. TAF15 and TUB served as compartment specific loading controls.
