Extended Data Fig. 1: Mapping of QTgw.cau-4B and nucleotide diversities of the genomic region spanning ZnF, EamA-B, and Rht-B1.
From: Reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat
a, Comparison of whole plants, spikes, and grain traits between the two parents, Shi and Heng. b, Significant quantitative trait locus (QTL) QTgw.cau-4B for grain traits mapped on chromosome 4B using (Shi × Heng) segregating population. The Y- and X-axes show the LOD value and genetic positions (cM) of markers, respectively. ‘Add’ and ‘R2’ represent additive effects and phenotypic variation explained by the QTL, respectively. c, QTgw.cau-4B mapped in the short arm of chromosome 4B and markers mapped nearby or within the QTL region. CEN, centromere. d, Selected six representative recombinants between markers M7 and ID-51 (red lines) flanking QTgw.cau-4B and their 1000-grain weight (TGW) data (right). Data are mean ± s.d. (n = numbers of biologically independent samples). P-values were calculated by a two-tailed Student’s t-test. e, Within the delimited QTgw.cau-4B region, six high-confident genes were annotated according to the Chinese Spring reference genome (IWGSC, RefSeq v1.1). f, Comparison of the genomic sequences between Shi4185 and Heng597 within the QTL region revealed a large fragment deletion (~500 kb) spanning three high confident genes including ZnF-B, EamA-B and Rht-B1 in Heng597. g, Nucleotide diversity of the genomic region spanning ZnF, EamA-B and Rht-B1 in wild emmer (WE, 28 accessions), domesticated tetraploid (DT, 93 accessions) and domesticated hexaploid wheat materials (HEX, 289 accessions). h, Microcollinearity analysis of the orthologs of ZnF, EamA and Rht-1 (or their orthologs) among different plant species.
