Extended Data Fig. 3: Related to Fig. 2. Further analysis of PXo localization.
From: A phosphate-sensing organelle regulates phosphate and tissue homeostasis
a,b,c,d,e,f,g, GFP staining of different organs from adult flies with ubiquitous expression of GFP-PXo for 5d. h,i,j,k,l,m,n,o, Co-staining of ubiquitously expressed GFP-PXo with various markers in the midgut, including Cnx99a (ER), RFP-KDEL (ER lumen), Hrs (early endosome), Rab7 (late endosome), ATP5A (mitochondria), GMAP (cis-Golgi), GalT-TagRFP (trans-Golgi), and WGA (glycans). 3D view is presented. p,q, P-Cho staining of midguts with ubiquitously expression of GFP-PXo for 5d and collected 2h post P-Cho injection. A non-injection group was included as the negative control. 3D view of confocal image is presented. r, Co-staining of ubiquitously expressed GFP-PXo with Avidin (ex vivo incubation). 3D view of confocal image is presented. s, Co-localization analysis with the quantification of Mander’s coefficient between GFP-PXo and Nile Red, ManII-TagRFP, or GalT-TagRFP. n = 4 midguts per group. P values from bottom to top: 1.16E-10, 1.14E-10. t, Shortest distance between each labeled PXo body and P-Cho (2h post injection), dextran (feeding), or avidin (feeding). n = 185 (P-Cho group), 149 (dextran), 122 (avidin) PXo bodies from 4 midguts were analyzed. P < 1E-14. Data are mean ± s.e.m. P values are from one-way ANOVA with Bonferroni’s multiple comparison test (s, t). Separate green and red channels are presented below merged images (h–r). Scale bars, 50 µm (a–g), 10 µm (h–r).
