Extended Data Fig. 3: Import kinetics in Pex13-WT and Y→S or F substitutions.
From: Peroxisome biogenesis initiated by protein phase separation
a, Percentage of cells displaying mCherry-SKL foci in the indicated strains following Pex5 induction. Error bars are mean ± s.e.m. for 3 independent experiments, n = 100 cells. The schematic on the right shows the Y→S or F substitutions made. b, Timelapse following mCherry-SKL foci formation in Pex13 WT cells (black arrow) and c, Pex13 S8 cells (orange and blue arrows). Scale bar 5 µm. d,e, mCherry-SKL foci fluorescence intensity of the cells imaged in panels b and c, respectively, plotted as a function of time after induction of Pex5 expression.
