Extended Data Fig. 7: UPP1 is expressed in PDA and TME cells and predicts survival outcome.
From: Uridine-derived ribose fuels glucose-restricted pancreatic cancer
a. RNAscope images showing UPP1 expression in tumour (PDA) compared to the adjacent non-tumour tissues. Pan-cytokeratin (PanCK) indicates the tumour cells; DAPI, nuclear stain. The images are representative of three 20x acquisitions per tissue slide, and of two independent experiments. Scale bar indicates 100 µm. b. UMAP plot showing the expression of UPP1 at the transcript level, as determined by single cell RNA seq of PDA tissues from two patients (#1238 and 1302). c. Violin plots showing UPP1 expression in various tumour microenvironment cell types, including myeloid and epithelial cells where UPP1 is highest. Right, UMAP plot showing the specific cell compartments expressing UPP1 for all patients’ samples combined (n = 16). Data used in plots b-c are from a previously published dataset55. d. Immunohistochemistry of UPP1 in patient biopsy sections from previously published tissue microarray48. Micrographs are representative from 213 patient samples in the microarray and two independent experiments. Large scale bar indicates 100 µm; scale bar on insets indicates 25 µm. e. Kaplan-Meier plot showing survival probability (log-rank test) based on UPP1 expression in three separate datasets. Each dataset was split into two – UPP1 high and UPP1 low – based on the ranked UPP1 expression value. Sample size: low = 133, high = 134 (ICGC); low = 62, high = 63 (GSE71729), low = 73, high = 73 (TCGA). TME – tumour microenvironment.
