Extended Data Fig. 7: Measurements of TAR-Tat:SEC binding using electrophoretic mobility shift assay (EMSA).
From: RNA conformational propensities determine cellular activity
Shown are EMSA binding curves for TAR bulge mutants U0,1,2,4,6,7 and UCU along with average apparent Kd values (see Methods) for each variant, obtained by fitting data to equation 2 using GraphPad Prism (version 9.3.1). Binding curves from 2 (U0, U2, U6, U7) or 3 (wt, U1, U4) independent experiments are overlayed (black: experiment 1, red: experiment 2, orange: experiment 3). Below the binding curves for each variant is one representative EMSA gel (experiment 1) of 2 total gels (U0, U2, U6, U7) or 3 total gels (wt, U1, U4) for each variant.
