Extended Data Fig. 6: Interaction between Gle2 and Rlp24 predicted by AlphaFold-Multimer.
From: Nuclear export of pre-60S particles through the nuclear pore complex
a, The structure of Gle2 contains seven WD40 domains. b, Surface electrostatic potentials of Gle2 displays a positively charged patch. c-d, The predicted Gle2-Rlp24 structure (d) and the associated PAE (predicted alignment error) value (c). Confidence level of PAE: Dark green is good (low error), light green is bad (high error). e, The predicted Gle2-Rlp24 structure shows that the negatively charged loop of Rlp24 is embedded into the positively charged patch of Gle2. f, Representative top5 models of the predicted Gle2-Rlp24 structures from S.cerevisiae (left column) and the top5 models of the predicted Gle2 and C-terminal of Rlp24 from H.sapiens (middle column) and M.musculus (right column), suggesting that a conserved negatively charged motif in the C-terminal of Rlp24 is responsible for binding to Gle2. g, Structural comparison of NPC-trapped pre-60S particle (left panel: skyblue, Rlp24: limon, Gle2: teal) and pre-60S-Drg1 complex (middle panel: lightblue, Drg1 hexamer: salmon). The positional relationship of Rlp24, Gle2 and Drg1 hexamer is enlarged in top view (left) and side view (right, central slice) at the bottom.
