Extended Data Fig. 10: Administration of ISRIB or NMN decreases the pathologic ISR activation observed in NDUFS2 cKO mice.
From: Mitochondrial integrated stress response controls lung epithelial cell fate
a–c, RNA-sequencing analysis of lung epithelial cells isolated from 35-day-old mice (WT n = 6; NDUFS2 control n = 21; NDUFS2 control + ISRIB n = 8; NDUFS2 control + NMN n = 11; NDUFS2 control/NDI1 n = 7; NDUFS2 cKO n = 13; NDUFS2 cKO + ISRIB n = 9; NDUFS2 cKO + NMN n = 11; NDUFS2 cKO/NDI1 n = 8 mice). Data in Fig. 4a-b were included in the analysis. Heatmaps of Ndufs2 and ISR signature gene transcripts (a). Enrichment plots of the ISR signature genes in lung epithelial cells from NDUFS2 cKO mice with vs. without ISRIB (b) (normalized enrichment score; −2.51, false discovery rate q value <0.0001), and NDUFS2 cKO mice with vs. without NMN (c) (normalized enrichment score; −2.39, false discovery rate q value <0.0001). d, Intracellular NADH/NAD+ ratios in lung epithelial cells from 35-day-old mice (NDUFS2 control n = 13; NDUFS2 control + ISRIB n = 8; NDUFS2 control + NMN n = 9; NDUFS2 cKO n = 7; NDUFS2 cKO + ISRIB n = 9; NDUFS2 cKO + NMN n = 9; WT n = 6 mice). p < 0.0001 by ANOVA. Adjusted p values by Šídák’s multiple comparisons test were provided in the graph.
