Extended Data Fig. 2: Characteristics of the distinct astrocyte transcriptional clusters and presence of cluster 7 glutamatergic astrocytes in all the hippocampal regions.
From: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS
a, UMAP representation of subset astrocytes labelled by transcriptionally distinct clusters (clustering resolution = 0.4, left) or datasets (right). b, Heatmap showing the total number of astrocytes for each dataset and each cluster. Noteworthy, cluster 7 was found in all interrogated hippocampal databases. c, Expression of the top 5 enriched genes per astrocyte cluster (see also Supplementary Table 2). d–g, Expression intensity per astrocyte cluster and corresponding dot plot for selected canonical astrocytic (d,e), vesicular trafficking, regulated exocytosis and glutamatergic pre-synaptic function markers (f,g). h, Heatmap showing the percent of cells in each cell cycle phase for each astrocytic cluster. i, Expression level per astrocyte cluster for Ifitm3, Vim, Megf10 and Merkt. j, High-magnification images of examples of glutamatergic astrocytes in various regions of the hippocampus: DGML: molecular layer of the dentate gyrus; Hilus: Hilus region of the dentate gyrus, CA3-RAD: stratum radiatum of the CA3 region, CA3-OR: stratum oriens of the CA3 region; CA1-RAD: stratum radiatum of the CA1 region, CA1-OR: stratum oriens of the CA1 region. The visualization was achieved using a combination of immunohistochemistry for tdTomato (Tom, red), GS/S100β (green), and DAPI (white) and fluorescent in situ hybridization for Slc17a7 (yellow), Slc17a6 (violet), Snap25 (blue), and Syt1 (pink). n = 12 slices, 2 mice. Scale bar: 10 µm.
