Extended Data Fig. 1: Single-cell multiomics reveals multiple TEC subsets with features of functionally defined parenchymal cells.

a, Flow cytometry plots of the gating strategy used to sort 60,000 EpCAM⁺CD45^– TECs followed by enrichment with 6,800 EpCAM⁺CD45^–IA-IE^loITGB4^lo/neg TECs from four pooled thymi of five-week-old B6.Adig mice for 10x Chromium Single Cell Multiome ATAC + Gene Expression assay. b, Frequency of 19 different subsets in the mouse thymic epithelial compartment as determined by scRNA-seq. Numbers above each bar indicate absolute counts out of 9,155 nuclei. c, UMAPs of selected gene expression per TEC subset as assayed by scRNA-seq. Each dot represents one nucleus. d, UMAPs of selected genes whose expression is enriched in hetreopost TEC subset as assayed by scRNA-seq. Each dot represents one nucleus.