Extended Data Fig. 3: R8s use histamine for motion detection and ACh for circadian photoentrainment.
From: A single photoreceptor splits perception and entrainment by cotransmission
a, Motion detection by R8s to slow (60°/s) visual stimuli. Pooled angular speed in norpAP41 (n = 10), R8-Gal4 (Rh5-Gal4,Rh6-Gal4)>norpA (n = 12), and norpAP41; R8-Gal4>norpA (n = 11) flies. Data are represented as mean (solid line) and s.e.m. (shading). b, Circadian photoentrainment by R8s. Phase plots of evening peaks of wild-type flies (left, n = 33), norpAP41;; cry02 mutant flies (second panel from left, n = 102), the flies with only R8s and H-B eyelets in dim-light detection (third panel from left, n = 93), and the flies with only R8s in dim-light detection (with H-B eyelets genetically ablated by Rh6-hid,rpr) (right, n = 73). c, Motion detection by R8s depends on histamine. Pooled angular speed of flies with Hdc-knockout in R8s (n = 11). d and e, Phase plots of evening peaks following genetic loss of ort (d, n = 91), or loss of both ort and ChAT (e, n = 74) in norpAP41;; cry02 flies with norpA rescued in R8s. f, R8-mediated motion detection does not depend on ACh from R8s. Pooled angular speed for R8-mediated motion detection after ChAT knockout in norpAP41 flies with norpA rescued in R8s (n = 9). Visual moving bars in a, c, and f: wave width of 30°, angular velocity of 60°/s, contrast of 100%, duration of 1 s. Pooled data: mean ± s.e.m.
