Extended Data Fig. 4: Morphological features of AMA neurons.
From: A single photoreceptor splits perception and entrainment by cotransmission

a, Postsynaptic candidates of R8s. Left, schematic of trans-Tango labeling; right, postsynaptic neurons of pR8s (Rh5-Gal4) and yR8s (Rh6-Gal4). b, VT037867-labled neurons overlap with R8s’ postsynaptic neurons that do not express ort. c, Single MCFO-labeled AMA neuron innervates the aMe, arborizes in multiple medullar visual columns, and exhibits an arcuate dorsal commissure. Left, an overview of the entire brain; middle, an expanded view of the aMe region; right, an expanded view of columnar arborization. Asterisk: cell body; dashed triangle: aMe; arrows: arborization in medulla columns; arrowhead: arcuate dorsal commissure. d, Single-cell morphology of the recorded AMA neurons. Single-cell morphology of a recorded AMA neuron, revealed by neurobiotin injection via the recording pipette after whole-cell recordings. Asterisk: cell body; dashed triangle: aMe; arrows: multicolumnar arborization; arrowhead: arcuate dorsal commissure. e, Multicolumnar arborization of AMA neurons. Top, RFP-labeled photoreceptors and a MCFO-labeled AMA neuron; bottom, an expanded view of the dashed boxes in top panels. f, Color inputs to AMA neurons. AMA neurons innervate pR8 columns (Rh5-eGFP, top), and yR8 columns (Rh6-eGFP, bottom). Arrowheads: overlap between an AMA neuron and R8s. Scale bar: 10 µm. g, Synapses between R8s and AMA neurons. Single-section image of GRASP signals between AMA neurons and pR8s (left) and yR8s (right).