Extended Data Fig. 1: Neutralizing antibody response after CoronaVac priming and one-dose variant spike boosting.
From: Repeated Omicron exposures override ancestral SARS-CoV-2 immune imprinting
a, Comparison of neutralizing titers between mice immunized with one doses of BA.5/BQ.1.1/XBB Spike protein and mice with 2 doses of CoronaVac followed by one-dose Spike protein boosters. The variants used for priming or boosting are indicated at the bottom of the figure and red, blue, yellow circles indicate the NT50s against BA.5, BQ.1.1, and XBB. b, c, Comparison of neutralizing titers among different groups of mice immunized with 2 doses of CoronaVac followed by one-dose BA.5/BQ.1.1/XBB Spike protein boosters administered with one-month, three-month, or six-month intervals between the second and third dose. b) Neutralizing titers against D614G; c) Neutralizing titers against variants that the mice boosted with. The variants used for priming or boosting are indicated at the bottom of the figure and red, blue, yellow circles indicate the NT50s against BA.5, BQ.1.1, and XBB. 10 mice were immunized and analyzed in each group (n = 10), except in b,c eight mice were immunized with BA.5 booster 6 months after priming (n = 8), and all neutralization assays were conducted in at least two independent experiments. Sera were collected four weeks after the last dose. Geometric mean titers (GMT) were labeled. All neutralization assays were conducted in at least two independent experiments. Statistical significance was determined using the two-tailed Wilcoxon rank sum test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and not significant (NS) p > 0.05.
