Extended Data Fig. 1: Diabetes-associated susceptibility to respiratory viral infection is associated with broadly impaired lung adaptive immunity.

a-m, WT (n = 16) and Akita mice (n = 7) infected with 50pfu PR8, analyzed at 10 d.p.i. a-b, Lung histology, two-sided Mann Whitney U-test. c, Lung CD4⁺ T cells, two-sided Mann Whitney U-test, lung CD8⁺ T cells, two-sided unpaired t-test. d, Lung Ki-67⁺CD8⁺ T cells, two-sided unpaired t-test. e, Lung IFNγ⁺CD8⁺ T cells, two-sided unpaired t-test. f, Lung T-bet⁺CD8⁺ T cells, two-sided Mann Whitney U-test. g, Lung FoxP3⁺CD4⁺ T cells, two-sided unpaired t-test. h, Lung B cells, two-sided Mann Whitney U-test. i, GC B cells, two-sided unpaired t-test. j-k, virus specific IgG2b, j, BAL, k, serum, two-sided Mann-Whitney U-test for EC50. l-m, virus-specific IgM, l, BAL m, serum, two-sided unpaired t-test for EC50. n-s, Mice infected with 50 pfu PR8, treated with insulin/PBS: WT+PBS (n = 16), WT+Ins (n = 15), Akita+PBS (n = 13), Akita+Ins (n = 15), pooled data from 2 experiments. n, Blood glucose. o, Lung CD4⁺ T cells, one-way ANOVA and Holm Sidak correction. p, Lung CD8⁺ T cells, Kruskal-Wallis and Dunn’s correction. q, Lung GC B cells, Kruskal-Wallis and Dunn’s correction. r-s, Virus-specific IgG2b, r, BAL, s, serum, Akita+PBS (n = 6), Akita+Ins (n = 9), one-way ANOVA with Tukey correction on EC50. All data mean+s.e.m. GC, Germinal centre.