Extended Data Fig. 4: Diabetes is associated with transcriptomic changes in respiratory viral infection.
From: Lung dendritic-cell metabolism underlies susceptibility to viral infection in diabetes
a-h, scRNA-seq of lungs of WT and Akita mice (n = 4/group) collected during steady-state,1 d.p.i. and 10 d.p.i. with 50pfu PR8. Quantification of key cell subsets, two-sided Wilcoxon test. Boxplots show 25th to 75th percentiles, the 50th percentile denoted by a thicker line; whiskers show 1.5× interquartile range max. or min. if smaller than 1.5× interquartile range. i-j, Heatmap with z-score of gene expression of differentially expressed genes between cells from WT and Akita, 10 d.p.i (n = 4/group). Differentially expressed genes identified by DESeq2 on pseudobulk counts in each population and adjusted p-value < 0.05. DESeq2 is based on Negative Binomial GLM fitting and Wald statistics with Benjamini and Hochberg procedure. i, downregulated, and j, upregulated genes in Akita mice. AT1, type 1 alveolar epithelial cell; AT2, type 2 alveolar epithelial cell.
