Fig. 5: Spatially resolved cell–cell communication.
From: A human embryonic limb cell atlas resolved in space and time
a, Dot plots showing expression (Z score) of ligands and cognate receptors in cell clusters (top), and heatmaps showing predicted cell-type abundance (bottom). b,d,f, Visium heatmaps of the hindlimb at PCW5.6 showing expression (normalized and log-transformed) of WNT5A (b,d), JAG1 (f) and their cognate receptors FZD10 (b), FZD4 (d) and NOTCH1 (f). The yellow stars indicate both the ligand and its receptor expressed. c,e,g, RNA-ISH expression of WNT5A (c,e), JAG1 (g) and their cognate receptors FZD10 (c), FZD4 (e) and NOTCH1 (g) in situ. Scale bars, 1 mm. h, Dot plots of FGFR2 expression and its ligands (top), and Visium heatmaps of a PCW6.2 human hindlimb showing spatially resolved selected mesenchymal cell cluster (separated by colour) signatures (bottom). i, Visium heatmaps of a PCW6.2 human hindlimb showing expression of FGF8, FGF10 and FGFR2. The yellow stars indicate that both FGF8 and FGFR2 are expressed. The white stars denote that both FGF10 and FGFR2 are expressed. j, RNA-ISH of FGF8, FGF10 and FGFR2 expression in the PCW6.2 hindlimb.
