Extended Data Fig. 2: The heterogeneity of epidermis and fibroblast.
From: A human embryonic limb cell atlas resolved in space and time
a, Uniform manifold approximation and projection (UMAP) visualization of AER-basal, basal and periderm cells. b, Violin plot showing the normalised and log-transformed expression level of WNT6, CYP26A1, SP8, ETV4 and FGF8 in AER-basal, basal and periderm cells. c, UMAP (left panel) and violin plot (right panel) showing the normalised and log-transformed expression level of FGF8 in the human limb. ProxMes, proximal mesenchyme; TenoProg, tendon progenitor; MyoProg, myogenic progenitor; MyoB, myoblast; MyoC, Myocyte. d, Heatmap across tissue section from PCW6.2 (post conception week 6 plus 2 days) human hindlimb showing FGF8 expression. e, RNA-ISH of tissue sections from human hind limb showing the expression pattern of FGF8. Scale bar, 500 μm. f, h, Dot plot showing the expression level of marker genes for selected fibroblast (Fibro) clusters (f) and muscle interstitial fibroblast (InterMusFibro) (h). The colour bar indicates the linearly scaled values of expression level. DermFibro, dermal Fibro; DermFibroProg, DermFibro progenitor. g, i, Heatmaps across tissue sections from PCW8.1 human hindlimb showing inferred abundance of each fibroblast cluster (g) and InterMusFibro (i). j, Immunofluorescence staining of MYH3 and ALDH1A3 on the skeletal muscle tissue (as also shown by H&E staining) from a PCW9 longitudinal section. n = 2. Scale bar, 50 μm.
