Extended Data Fig. 2: circFAM53B did not influence tumour cell activities.
From: Tumour circular RNAs elicit anti-tumour immunity by encoding cryptic peptides
MCF-7 cells were transfected with siGFP, circFAM53B siRNA-1 and siRNA-2 (sicirc-1 and sicirc-2, respectively) and MDA-MB-231 cells were transfected with empty vectors (vec) and circFAM53B overexpressing plasmid. (a, b) Relative quantitation of circular (a) and linear FAM53B levels (b) by RT-qPCR is shown. (c) CCK-8 assays were used to detect viability of MCF-7 and MDA-MB-231 cells after transfection. (d) Flow cytometric analysis of Annexin V/ PI staining was used to detect apoptosis of MCF-7 and MDA-MB-231 cells after transfection. Representative flow cytometric plots. Numbers represent the proportion of annexin V+ cells. (e, f) Migration and invasion assays of MCF-7 (e) and MDA-MB-231 cells (f). Scale bars, 100 μm. (g) The levels of epithelial-mesenchymal transition markers (E-cadherin and vimentin) in MCF-7 and MDA-MB-231 cells after transfection, as detected using western blotting (n = 3 independent experiments). For gel source data, see Supplementary Fig. 3. Results are mean ± s.d. of n = 3 (a-f) independent experiments producing similar results. ****P < 0.0001 compared with untreated cells (-) (a). P values were determined by two-tailed one-way ANOVA with Dunnett’s multiple-comparisons test (a-f).
