Extended Data Fig. 5: Deciphering the variability of active GI hubs.
From: RNA-mediated symmetry breaking enables singular olfactory receptor choice
(a) GI hubs encompassing the active P2 allele in 3 gg8tTA>tetOP2 representative nuclei. (b) Binary arrays generated for each cell (rows) depict GIs within <2.5 p.r. of the mor28 allele (non-Cas, chr14) when it is active (top, mor28-i-GFP, n = 74 cells), or inactive (bottom, OMP-i-GFP, n = 40 cells). (c) 36 mor28iGFP nuclei and 42 gg8tTA>tetOP2iGFP nuclei were compared between themselves and each other for overlap in the combination of trans GIs in their active GI hub (mor28-vs-mor28, n = 630 cell pairs; P2-vs-P2, n = 861 cell pairs; mor28-vs-P2, n = 1453 cell pairs). GIs from chr7 and chr14 are excluded from this analysis to examine GIs that are trans to both P2 and mor28. (d) Hierarchical clustering of GI spatial relationships was performed on Dip-C models to extract the relative size of the active GI hub (5.42 ± 3.00 GIs, n = 161) vs. inactive GI complexes (2.39 ± 1.84 GIs, n = 7,990) across all cells. (e) Hierarchical clustering of GI spatial relationships in a 2 gg8tTA>tetOP2 and 2 mor28-i-GFP nuclei.
