Extended Data Fig. 1: Greek Island inactivation during the transition to transcriptional singularity.
From: RNA-mediated symmetry breaking enables singular olfactory receptor choice
(a) Two separate multiomes were constructed from independent mice and analyzed separately (see Fig. 1). UMAP representation of the mouse MOE multiome, constructed from weighted nearest neighbors analysis on RNA and ATAC data from 9,034 quality-controlled cells from one mouse. The neuronal lineage contains globose basal cells (GBCs), immediate neuronal precursors (INPs), immature (iOSNs) and mature olfactory sensory neurons (mOSNs). (b–c) Pseudotime projection onto the neuronal lineage was verified with the expression of known marker genes (scaled mean ± SEM, n = 9,034 cells from one multiome). (d) Cumulative GI accessibility dynamics, averaged for all cells rounded to the nearest pseudotime, separate into 3 phases: early, from GBC to INP3; mid, from INP3 to iOSN; and late, mOSN (mean ± SEM per pseudotime, n = 2,371 cells from one multiome). (e) GI accessibility over pseudotime per GI. (f) Cumulative GI accessibility over pseudotime plotted against cumulative accessibility of OR promoters (cinnamon, n = 1255 sites, mean ± SEM), (g) expression level of the most highly expressed OR per cell (blue, SCT normalized counts, mean ± SEM), (h) accessibility per active GI (green), (i) top mOSN cCREs (pink, n = 71 sites, mean ± SEM), and (j) cumulative accessibility of Lhx2 & EBF1 bound sites in mOSNs (red, n = 4793 sites, mean ± SEM per pseudotime). (k) Boxplots comparing fraction of cells with accessibility in mOSN cCREs (27.9% ± 10.9%, n = 71), GIs (5.70% ± 4.94%, n = 63) and OR promoters (3.66e-2% ± 1.36e-1%, n = 1255) in mOSNs. Each boxplot ranges from the upper and lower quartiles with the median as the horizontal line and whiskers extend to 1.5 times the interquartile range. (l) 326,836 unique cell pairs, from 809 mOSNs, were tested for overlap in their accessible GIs. (m) Average frequency of an OR (mean ± SEM), between all unique cells making up cell pairs sharing between 0 and 7 GIs. The dashed red line represents the expected average OR frequency if the number of unique ORs were to equal the number of unique cells (n = 809 cells from one multiome). (n) Frequency of each OR expressed by all unique cells making up cell pairs sharing between 0 and 7 GIs, colored by OR identity.
