Extended Data Fig. 4: Determining the dimensions of the GI hub.
From: RNA-mediated symmetry breaking enables singular olfactory receptor choice
(a,b) Representative models of the whole genome (left), OR genes and GIs (middle), and the active GI hubs (right) are shown for 4 gg8tTA>tetOP2 and mor28-i-GFP nuclei colored by chromosome. (c,d) Left, line plots depict the mean ± SEM number of trans Greek Islands, normalized to radial distance, at binned distances away from P2 gene or mor28 gene on non-Cas (green, active in GFP+ cells) or Cas (red, always inactive) chromosomes. Right, heatmaps depict the number of trans Greek Islands, normalized to radial distance, at binned distances away from Olfr17 or Olfr1507 alleles for each individual cell in a dataset. Dip-C was performed over 2 independent experiments on FAC-sorted GFP+ cells pooled from gg8tTA>tetOP2 mice and mor28-i-GFP mice to generate a total of 161 high quality cells (Welch two-sample t-test; gg8tTA>tetOP2, P2 locus, 2.5 p.r., p = 3.4e-8; gg8tTA>tetOP2, P2 locus, 5 p.r., p = 3.1e-5; gg8tTA>tetOP2, P2 locus, 10 p.r., p = 0.03; mor28-i-GFP, mor28 locus, 2.5 p.r., p = 0.036; mor28-i-GFP, mor28 locus, 10 p.r., p = 0.017; n = 87 P2 cells and n = 74 mor28 cells).
