Fig. 5: Engram neurons in mPFC and BLA share transcriptional machinery in consolidating remote memory.
From: Spatial transcriptomics reveal neuron–astrocyte synergy in long-term memory
a–g, Analysis of scRNA-seq data. a, Cellular composition of BLA and mPFC. b, Integrated clustering of BLA and mPFC neurons, coloured by region. c, DEGs of TRAPed cells of EX.Znt3 (left), Int.Vip (middle) and EXT.Syt6 (right). The x axis shows fold change of FR over NF in BLA and the y axis shows the fold change of mPFC. Significant DEGs are shown in orange. P < 0.05 for both conditions (axes); two-sided Mann–Whitney–Wilcoxon test. d, Quantification of significant DEGs in neuron clusters 1–3. e, DEGs (FR over NF, TRAPed cells) from BLA and mPFC among B-P.EX.Znt3, B-P.Int.Vip, and B-P.EX.Syt6 neurons. f, Nts expression in tdT+ B-P.EX.Syt6 neurons from BLA. Two-sided Mann–Whitney–Wilcoxon test. g, Ntsr2 expression in all cells from BLA, Ntsr2 expression is highly enriched in astrocytes.
