Extended Data Fig. 5: Effect of spermidine on Z-DNA formation and thymic cell populations.
From: AIRE relies on Z-DNA to flag gene targets for thymic T cell tolerization
a, Density plot showing the effect of spermidine vs control PBS injection on Z-DNA intensity in mTECs measured by flow cytometry using an anti-Z-DNA antibody. b, Representative cytofluorimetric plots and quantitative summary for mTECs of WT and Aire-KO mice treated with spermidine or control PBS. c, Cytofluorometric gating scheme for analyses of thymocyte compartments. d, Analogous plots to panel b for thymocyte compartments. Error bars, mean ± s.e.m. from n = 3 biological replicates. KO: Aire-KO.
