Extended Data Fig. 6: Bulk RNA-saq of iMG and phenotypic assays.
From: APOE4/4 is linked to damaging lipid droplets in Alzheimer’s disease microglia
a, Volcano plot of differential gene expression analysis of untreated and Aβ treated APOE4/4 iMG. b, Volcano plot of differential gene expression analysis of lipid droplet high and lipid droplet low APOE4/4 iMG. c, Volcano plot of differential gene expression analysis of lipid droplet high and lipid droplet low APOE3/3 iMG. d, Volcano plot of differential gene expression analysis of lipid droplet high APOE3/3 iMG and lipid droplet high APOE4/4 iMG. e, Example gating scheme for separating cells based on lipid droplet content. f, LysoTracker area per cell after incubation with fAβ in APOE3/3 and APOE4/4 iMG (n = 3 replicate wells per condition; one-way ANOVA; mean ± s.e.m.). g, Phrodo zymosan phagocytosis area per cell after incubation with fAβ in APOE3/3 and APOE4/4 iMG (n = 3 replicate wells per condition; one-way ANOVA; mean ± s.e.m.). h, Normalized gene expression counts for significant DEGs in LD-high versus LD-low iMGs (n = 2 replicate wells per condition). i, Secreted chemokines in LD-high versus LD-low iMGs (n = 2 replicate wells per condition).
