Extended Data Fig. 5: Astrocyte Acly signaling and ACLY + p300+ astrocytes in EAE.
From: Disease-associated astrocyte epigenetic memory promotes CNS pathology
(a) Immunostaining (left) and quantification (right) of H3K27ac+ and ACLY+ astrocytes from sgScrmbl- and sgAcly- transduced mice at 21days after EAE induction (n = 6 spinal cord sections; n = 3 mice per group). Unpaired two-sided t-test. (b) Staining with FluoroMyelin dye and percentage of myelin loss in spinal cord of sgScrmbl- and sgAcly-treated mice (n = 9 spinal cord sections; n = 3 mice per group). Lesions indicated by arrowheads. Unpaired two-sided t-test. (c) Quantification of CNS-resident cells from sgScrmbl- and sgAcly-transduced mice (n = 5 per group). Unpaired two-sided t-test. (d,e) Analysis of CNS T cells (up) and splenic T cells (bottom) from sgScrmbl- or sgAcly-transduced mice (n = 5 per group). Unpaired two-sided t-test. Data shown as mean ± s.e.m. (f) Immunostaining (left) and quantification (right) of ACLY+SOX9+, p300+SOX9+, and ACLY+p300+SOX9+ astrocytes in EAE and control mice (n = 6 spinal cord sections; n = 3 mice per group). Unpaired two-sided t-test. Data shown as mean ± s.e.m.
