Extended Data Fig. 7: ACLY and p300 signaling in NOD EAE astrocytes.
From: Disease-associated astrocyte epigenetic memory promotes CNS pathology
(a) Immunostaining (left) and quantification (right) of H3K27ac+ and p300+ astrocytes from sgScrmbl- and sgEp300- transduced NOD mice 64 days after EAE induction (n = 6 spinal cord sections; n = 3 mice per group). Unpaired two-sided t-test. (b) Immunostaining (left) and quantification (right) of H3K27ac+ and ACLY+ astrocytes from sgScrmbl- and sgAcly- transduced NOD mice 64 days after EAE induction (n = 6 spinal cord sections; n = 3 mice per group). Unpaired two-sided t-test. (c) Quantification of CNS-resident cells from sgScrmbl-, sgEp300, and sgAcly-transduced mice (n = 7 per group). Unpaired two-sided t-test. (d,e) Analysis of CNS T cells (up) and splenic T cells (bottom) from sgScrmbl-, sgEp300, and sgAcly-transduced mice (n = 4 per group). Unpaired two-sided t-test. Data shown as mean ± s.e.m.
