Extended Data Fig. 6: The C9 acylation of 12 in the presence of exogenously supplemented 2MB acid.
The conversion of 12 to 19 was limited possibly due to the limited availability of intracellular 2MB acid. Increased concentration of 2MB added to the culture medium effectively improved the conversion and the concentration of 2MB supplementation is thus determined to be 500 mg l−1. Data are mean ± s.d.; n = 3 biologically independent samples. *P value < 0.05. Student’s two-tailed t-test. More statistical analysis is available in the source data file.
