Extended Data Fig. 7: Tectal subtype labelling does not bias larval sleep amount and sleep-wake states have non-uniform effects on synapses within neuronal compartments.
From: Sleep pressure modulates single-neuron synapse number in zebrafish
a, Schematic of behavioural and synapse tracking experiment set up. Larval locomotor behaviour was tracked on a 14 h–10 h LD cycle from 6–8 dpf. The average activity ( ± 95% CI) of 10 example larvae are plotted across two days and nights. Larvae were removed from the tracking arena and imaged at lights on (ZT0) and again at ZT10 (dotted red bars). White and grey boxes indicate day and night periods, respectively. b, 7 dpf Larvae had similar levels of sleep and sleep bout lengths at night ( ± SEM) regardless of the FoxP2.A tectal neurons subtype labelled in each larva (ns, p > 0.05, Kruskal-Wallis; 5 independent experiments). c, For each neuron/larva, the average percentage change of synapse number is plotted versus the average 7 dpf night-time sleep. d, Type 2 tectal neurons were divided into four segments: the primary neurite, proximal arbour, inter-arbour area, and distal arbour. e, The average and 68% CI of synapse number and intensity dynamics within each of the four segments. Grey lines represent segments from individual neurons. *P = 0.037, repeated-measures ANOVA with Greenhouse-Geisser correction. f, Proximal and distal arbours synapse number dynamics are not correlated. The relationship between the absolute and relative (%) synapse number change of the proximal and distal arbours of individual Type 2 neurons during the day and night phase. Linear regressions in c and f are fitted with 95% CI.
