Extended Data Fig. 5: FoxP2.A tectal neurons have four morphological subtypes.
From: Sleep pressure modulates single-neuron synapse number in zebrafish
a, Principal component analysis using the subtype morphological features depicted in Fig. 2a. Four principal components (dotted line) account for >85% of the variance. b, The optimal number of clusters for k-means clustering was determined using the elbow method by plotting the within-cluster sum of squares. Four clusters were chosen (dotted line). c, The six features used to cluster FoxP2.A neurons (collected over 26 experiments) by morphological subtype. Boxes depict the median and interquartile range and the whiskers represent the distribution for each parameter. The slashed zero means the feature is absent. d-f (left), Synapse counts with 68%CI (d), average change (68%CI) in synapse counts (e), and percentage change (68%CI) in synapse counts (f) in different FoxP2.A tectal neuron subtypes of larvae raised in normal LD conditions. d-f (right), Each neuron is plotted, grouped by subtype. g, Average (68%CI) synapse counts of tectal subtypes (left) and for each n= neuron (right) across multiple days under clock-break (LL) conditions. Note the lack of Type 2 neurons in LL. h, Average (68%CI) synapse counts during the subjective day or night under clock-break conditions. i, Average change (68%CI) in synapse counts (left) and single neurons (right) across multiple days under clock-break conditions, sorted by tectal subtype. j, the average change (68%CI) in synapse counts for the subjective day and night under clock-break conditions. Data in g-j are from 4 independent experiments.
