Extended Data Fig. 7: Immunofluorescence analysis of iNOS+ cells at the implant-tissue interface.

a, Representative immunofluorescence images at the adhesive implant-tissue interface on day 3 post-implantation to the abdominal wall. b, Quantification of iNOS + /neutrophil elastase+ and iNOS + /CD68+ cells per unit area on day 3 post-implantation for the adhesive implant-tissue interface. c, Representative immunofluorescence images at the non-adhesive implant-tissue interface on day 3 post-implantation to the abdominal wall. d, Quantification of iNOS + /neutrophil elastase+ and iNOS + /CD68+ cells per unit area on day 3 post-implantation for the non-adhesive implant-tissue interface. In immunofluorescence images, cell nuclei are stained with 4′,6-diamidino-2-phenylindole (DAPI, blue); green fluorescence corresponds to the expression of macrophage (CD68) and neutrophil (neutrophil elastase); red fluorescence corresponds to the expression of iNOS. *In images indicates the implant; white dotted lines in images indicate the implant-tissue interface. Values in b,d represent the mean and the standard deviation (n = 3 implants; independent biological replicates). Statistical significance and P values are determined by two-sided unpaired t-tests; ns, not significant; **P ≤ 0.01.