Extended Data Fig. 1: High Fat Diet feeding induces an obese phenotype.
From: Obesity induces PD-1 on macrophages to suppress anti-tumour immunity
a, Mouse body weights during 20 weeks of LFD or HFD feeding prior to MC38 tumor injection (n = 20 mice per diet). b, Mouse food intake during 20-week diet treatment. Food weighed weekly per cage and intake calculated as food weight difference between weeks (difference in food weight(g)/7 days/5 mice per cage X kcal of diet) (45% HFD = 4.73 kcal/g; LFD = 3.85 kcal/g) (n = 4 cages with 5 mice per cage). c-d, Blood glucose and blood insulin levels measured via tail bleed following a 4-hour fast after 20 weeks of diet treatment (n = 20 mice per diet). e-h, Representative tumor growth curves of mice inoculated with 5 × 105 MC38 cells following 20 weeks of diet treatment (n = 6-8 mice per group). i, Tumor weights on day 19 following subcutaneous inoculation. “X” indicates days of anti-PD-1 or IgG injection. Data points identified as “+” were pooled together at equal amounts of live cells for subsequent single cell RNA sequencing analysis. j, Percent change in tumor growth in response to a-PD-1 treatment within respective diets ((Tumor Diameter Day 9 LFD α-PD-1 – Diameter Day 19 LFD IgG) ÷ Diameter Day 19 LFD IgG × 100). P values were calculated using an unpaired two-tailed t-test (a-d, j) or two-way ANOVA using a Fishers LSD test for multiple comparisons (i). ns=p > 0.05; * p ≤ 0.05; ** p ≤ 0.01.
