Extended Data Fig. 9: Characterization of the inhibition type of atomoxetine, desipramine, bupropion, and escitalopram.
From: Molecular basis of human noradrenaline transporter reuptake and inhibition
a, The [3H]-NE uptake assays by NET in the absence (0 nM in yellow) or presence (30 nM in green and 120 nM in rose red) of atomoxetine. The data were fitted using the Michaelis–Menten non-linear fitting method. Curves were calculated from three biologically independent experiments: one experiment with three technical repeats and two experiments with two technical repeats (mean ± SEM; n = 7). Non-linear regression fits show characteristic results for competitive inhibition of NET by atomoxetine. b-d, The [3H]-NE uptake assays revealed inhibition mechanism for desipramine, bupropion, and escitalopram. The results are illustrated with yellow curves to represent the absence of inhibitors (WT), green curves for medium inhibitor concentration, and rose red curves for high inhibitor concentration. The data were fitted using the Michaelis–Menten non-linear fitting method. Curves were calculated from three biologically independent experiments: one experiment with three technical repeats and two experiments with two technical repeats (mean ± SEM; n = 7). Non-linear regression fits show characteristic results for non-competitive (mixed-type) inhibition of NET by desipramine, bupropion, and escitalopram.
